Skip to content

FREE 24-HOUR DELIVERY OVER £75

All researchProtocols

Using the Peptide Reconstitution Calculator

A step-by-step guide to using the peptide reconstitution calculator for accurate concentration preparation.

Scientific background / literature overview only. Discussion of published research does not describe the intended use of Novex Peptide products.

Novex Peptide Research Team1 August 2026 5 min read
Using the Peptide Reconstitution Calculator

Introduction

> **Research Context Notice** — This article summarises published scientific literature for educational and research-reference purposes only. Discussion of experimental, preclinical or clinical findings does not represent a permitted use of Novex Peptide products. Products supplied by Novex Peptide are intended exclusively for in-vitro laboratory research and are not intended for human or animal administration.

The laboratory concentration calculator is a laboratory tool designed to help researchers determine the correct volume of solvent needed to achieve a target peptide concentration. This guide explains how to use it effectively.

How the Calculator Works

The calculator uses the basic formula:

**Concentration (mg/mL) = Mass of peptide (mg) ÷ Volume of solvent (mL)**

Given any two of these values, the calculator determines the third.

Using the Calculator

Step 1: Enter Peptide Mass

Enter the mass of the peptide in your vial. This is printed on the product label and the COA (e.g., 5mg, 10mg).

Step 2: Enter Desired Concentration

Enter your target concentration in mg/mL. Common concentrations for laboratory research range from 1 mg/mL to 10 mg/mL, depending on the experimental protocol.

Step 3: Calculate Solvent Volume

The calculator determines the volume of solvent (in mL or µL) needed to achieve the target concentration.

Step 4: Reconstitute

Using a calibrated laboratory micropipette, add the calculated volume of solvent to the vial following proper laboratory reconstitution technique.

Common Concentrations

|---|---|---|

Volume Conversion

For laboratory micropipette work, volumes may be expressed in microlitres (µL):

  • 1 mL = 1000 µL
  • 0.5 mL = 500 µL
  • 0.1 mL = 100 µL
  • Tips for Accurate Reconstitution

  • Use calibrated laboratory micropipettes for precise volume measurement.
  • Account for the dead volume in the vial — a small amount of solution cannot be withdrawn.
  • Verify the peptide mass on the COA, not just the label.
  • Use the correct laboratory solvent for your peptide (bacteriostatic water, sterile water, or buffer as appropriate).
  • Research Limitations

    For laboratory concentration and aliquot calculations only. This tool does not provide dosing, administration or human-use guidance. Novex Peptide does not provide dosing, administration, or injection guidance. All products are for in-vitro laboratory research only.

    References

    1. 1.Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th ed. WH Freeman; 2002.
    2. 2.Guide to Peptide Reconstitution. Bachem Technical Notes. 2019.

    Research use only. This article is for educational purposes only and does not constitute advice for any application involving human use. No medical claims are made.

    Share

    Stay informed on new research

    Join our newsletter for new research articles, batch updates, and educational content. No medical advice. Unsubscribe anytime.